Map of Experiments for CTL Flow Cytometry Assay
2015-01-13azim58 - Map of Experiments for CTL Flow Cytometry Assay
- Gel of OVA plasmid 6-13-11
- Initial 3T3 cell culture 6-20-11
- Test of caspase kit 10-12-11
- Protein boost with OVA 10-13-11
- Flow cytometry 10-13-11
- Caspase kit test (2nd time) 10-17-11
- Fresh media for targets and effectors 10-23-11
o T cell enrichment 10-26-11
o Caspase Staining 10-26-11
o Flow Cytometry to analyze cells
- Ova Immunization ELISA 10-25-11
- NIH 3T3 Apoptosis 11-2-11
o Cell Staining 11-10-11
o T Cell purification 11-21-11
- IL-2 and Peptide Addition 12-7-11
- Killing Assay with Caspase 12-13-11
- T cell assay maintenance
- Killing Assay with Annexin V 12-21-11
- Passaged 3T3 and CRL2116 cells 1-3-12
- Preparation for T cell purification comparison 1-4-12
- T Cell purification to compare old T cell enrichment kits 1-16-12
- peptide preparation 1-23-12
- peptide plate transfer 1-23-12.2216
- Ova protein mouse immunization 1-26-12
- test of decanting after fluor is added 2-3-12
- ELISPOT w/Ova splenocytes 2-9-12
- ELISPOT w/Ova splenocytes 2-11-12
- Imaged ELISPOT plates 2-13-12
- SIINFEKL Peptide Incubation 3-13-12
- ELISPOT w/Ova Peptide 3-14-12
- Ova protein boost 3-30-12
- ova splenocyte test 4-6-12
- killing assay 4-8-12
- bone marrow surgery 6-7-12
- Ova Bullet preparation 6-18-12
- Ova Protein Boost 7-3-12
- Ova ELISA 7-13-12 (Ova ELISA 7-13-12)
- B16F10 Cell Culture 7-17-12
- chromium release assay 8-17-12 to 8-18-12
- CRA with more E:T ratios 8-21-12
- SIINFEKL dilution 9-26-12
- Ova protein boost 10-2-12
- Ova CTL Assay 10-8-12
- Ova mouse immunization 10-15-12
- CRA parameter test 10-18-12
- ova ctl assay w/o tissue culture flask 10-20-12
- CRA Parameter Test 10-18-12
- Ova CTL Assay w/o tissue culture flask 10-20-12
- aged-young cr release assay 1-4-13
- obtained splenocytes from injured mice 1-6-13
- aged-young cr release assay 1-10-13