Is there any reason to scan nitrocellulose with pe instead of agilent_

2015-01-13

azim58 - Is there any reason to scan nitrocellulose with pe instead of
agilent?


The answer appears to be yes. The forward scanning perkin elmer slides do
exhibit a higher ratio when comparing undiluted lysate with empty cell
lysate.

This data is from the 11-3-11 nitrocellulose run.

(undilitued median intensity)/(empty cell lysate median intensity)
PE Scan
24942/6967 = 3.58
20112/6082 = 3.31

Agilent Scan
6649/2457 = 2.71
6924/2650 = 2.61