Investigation 6-23-13d1107

2015-01-13

azim58 - Investigation 6-23-13d1107


TR_E10339
f
catcagcttccacaggtttc
r
cgatggttaagatgaatccg


355 bp expected with TR_E10339F and TR_E10339R primer with Shen's
sequence. Hojoon's table indicates that the expected size is 315 bp.

protein produced by thap2+tmem19 fusion
MPTNCAAAGCAATYNKHINISFHRFPLDPKRRKEWVRLVRRKNFVPGKHTFLCSKHFEASCFDLTGQTRRLKMDA
VPTIFDFCTHIKSLVTFGLFLRGAGCSPSSFLL

amino acid sequence around fusion
IKSLVTFGLFLRGAGCSPSSFLL

amino acid sequence starting at fusion
VTFGLFLRGAGCSPSSFLL

Therefore, this sequence that Shen gave me would produce the same
sequence as the 1st peptide sequence given in Hojoon's table for Thap2