In-Fusion SMARTer cDNA Library Manual
2015-01-13azim58 - In-Fusion SMARTer cDNA Library Manual
In-Fusion SMARTer cDNA.pdf
and here
"C:\kurt\storage\CIM Research Folder\DR\2013\3-13-13\In-Fusion SMARTer
cDNA.pdf"
Sequencing primers
Forward Screening Primer (10 μM)
TCACACAGGAAACAGCTATGA
Tm of 50.3 according to oligomaster spreadsheet
Reverse Screening Primer (10 μM)
CCTCTTCGCTATTACGCCAGC
Tm of 56.2 according to oligomaster spreadsheet
The M13 forward and reverse primers can also be used
M13 Forward Sequencing Primer (Sense Strand): 5'-GTAAAACGACGGCCAGT-3'
M13 Reverse Sequencing Primer (Antisense Strand):
5'-AGCGGATAACAATTTCACACAGGA-3'
SMARTer IIA Oligonucleotide Sequence as determined by sequencing
AAGCAGTGGTATCAACGCAGAGTACATGGG
Other information
pSMART2IF
Transcript orientation question 6-23-13