ImageJ by NIH

2015-05-11

azim58 - ImageJ by NIH
ImageJ portal
Fiji portal

ImageJ by NIH


total intensity in an area in ImageJ
Analyze->Measure then Results->Set Measurements->Integrated Density and
get the RawIntDen (raw intensity density): the sum of the values of the
pixels in the image or selection

see Digitally Counting Colonies
see Convert Image to Numbers for Each Pixel


Advanced program built on top of ImageJ 


Fiji is Just ImageJ


Fiji is an image processing package. It can be described as a "batteries-included" distribution of ImageJ (and ImageJ2), bundling Java, Java3D and a lot of plugins organized into a coherent menu structure. Fiji compares to ImageJ as Ubuntu compares to Linux.





http://fiji.sc/Fiji



Color picker method in ImageJ