Ideal results of ds cDNA Synthesis
2015-01-13azim58 - Ideal results of ds cDNA Synthesis
Basically a moderate strong smear from 0.5 to 5 kb should be detected.
A good overview of what a 2nd strand synthesis looks like can be found in
the SMARTer cDNA library construction kit manual
In-Fusion SMARTer Directional cDNA Library Construction Kit
Here is the relevant page from the manual
"C:\kurt\storage\CIM Research Folder\DR\2013\1-28-13\cDNA\Typical results
of PCR and ds cDNA Synthesis.pdf"