High throughput proteomic strategies for identifying tumour-associated antigens. paper
2015-01-13azim58 - High throughput proteomic strategies for identifying
tumour-associated antigens. paper
High throughput proteomic strategies for identifying tumour-associated
antigens. paper
Gunawardana CG, Diamandis EP.
Cancer Lett. 2007 Apr 28;249(1):110-9. Epub 2007 Feb 15. Review.
2007
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital,
Toronto, Ont., Canada.
article is accessible
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Folder\DR\2013\1-26-13\some_serex_papers\High throughput proteomic
strategies for identifying tumour-associated antigens..pdf"
Notes
High throughput proteomic strategies for identifying tumour-associated
antigens
University of Toronto Canada
2007
Cancer Letters Elsevier
Serological analysis of recombinant cDNA expression libraries (SEREX)
q
The development of SEREX 8 in the 90s offered
a high-throughput approach to analyze the humoral
response against TAAs in cancer patients.
pros
avoid artifacts of cultured cells
con
antibodies to non-tumor associated antibodies
q
A database
(http://www2.licr.org/CancerImmunomeDB/), Cancer
Immunome Database, contains all the autoantigens
identified by SEREX.
===========================================================================
SERPA
2D western blot -> mass spec
pro
fast
post-translational modifications
con
limited material
co-migrating proteins
issues with reproducibility
===========================================================================
protein microarrays
q
‘‘immunome’’ of a cancer
biased approach (with known analytes) (autoantibody or autoantigen)
Invitrogen protoArray prospector
unbiased approach (new analytes can be discovered) (phage display or cell
lysate)
Invented in 1985 by Smith 21, phage display is a
powerful proteomic tool used to express proteins or
domains of proteins.
pro
high throughput
a lot of data
con
cost
pattern recognition software
q
The Luminex xMAP technology
appears to be very promising as 100 or more
analytes can be studied in one sample alone.
^this is an automated ELISA system