HBSS

2015-01-13

azim58 - HBSS


HBSS (Hanks’ balanced salt solution)
  1. 40 g KCl (5.4 mM final)
  2. 09 g Na2HPO4⋅7H2O (0.3 mM final)
  3. 06 g KH2PO4 (0.4 mM final)
  4. 35 g NaHCO3 (4.2 mM final)
  5. 14 g CaCl2 (1.3 mM final)
  6. 10 g MgCl2⋅6H2O (0.5 mM final)
  7. 10 g MgSO4⋅7H2O (0.6 mM final)
  8. 0 g NaCl (137 mM final)
  9. 0 g D-glucose (5.6 mM final)
  10. 2 g phenol red (0.02%; optional)

Add H2O to l liter and adjust pH to 7.4 with 1 M HCl or 1 M NaOH
Filter sterilize and store up to 1 month at 4°C
HBBS can be purchased from Biofluids or Bio-Whittaker.
HBSS may be made or purchased without Ca2+ and Mg2+ (CMF-HBSS). These
components
are optional and usually have no effect on an experiment; in a few cases,
however, their
presence may be detrimental. Consult individual protocols to see if the
presence or absence
of these components is recommended.
Bottles should be kept tightly closed to prevent CO2 loss and subsequent
alkalinization.

This information can also be found here:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Application
s/Cell-Culture/Mammalian-Cell-Culture/reagents/Balanced-Salt-Solutions/hbss