Gel extraction of SMC related bands 6-24-13

2015-01-13

azim58 - Gel extraction of SMC related bands 6-24-13


These bands were gel extracted on 6-22-13

Gel image here
"S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor
cDNA Library\6-22-13\chimeric transcript gel 0p7 exp inverted 6-22-13.jpg"


Quantification of bands 6-24-13d1006

Now that I have quantified the bands I can figure out how to setup the
pJET cloning reaction.

Need to know how much DNA corresponds to 0.15 pmol ends for all 3 bands

  1. 15 pmol ends of top band. I would say the top band is around 460bp
(0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")
/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(460*"bp")/(1*"molecule")*(1*"m
ol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL
")/(1.8*"ng")=12.7*"uL"

  1. 15 pmol ends of middle band. I would say the middle band is around 160
bp
(0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")
/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(160*"bp")/(1*"molecule")*(1*"m
ol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL
")/(0.8*"ng")=9.9*"uL"



  1. 15 pmol ends of bottom band. I would say the middle band is around 140
bp

(0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")
/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(140*"bp")/(1*"molecule")*(1*"m
ol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL
")/(0.6*"ng")=11.6*"uL"