Freezing Tissue Culture Mammalian Cells
2015-01-13azim58 - Freezing Tissue Culture Mammalian Cells
Pellet the cells and resuspend in 1 mL freezing media in a cryogenic
vial. Place the vial into the cryogenic container containing isopropanol
(such a container allows the temperature to drop slowly) and place this
container into -80 C overnight. Transfer vial to -150 C for long term
storage.
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Cryogenic vials last seen in tissue culture room in 2nd or 3rd drawer in
dresser near door.
Tissue culture freezing media (some amount of DMSO and FBS?)