Flow Cytometry CTL Protocol

2015-01-13

azim58 - Flow Cytometry CTL Protocol

Overall flow cytometry CTL assay diagram

Flow Cytometry CTL Protocol

Mouse Immunization
Surgical Removal of Spleens
Splenocyte Preparation Protocol
Induction of Cytolytic Activity in CTL Precursors
Purification of T cells with Robosep Kit
Killing Assay
Killing Cells with Camptothecin for Positive Control
Caspase Kit
Annexin V protocol
Annexin V and PI or 7-AAD protocol
7-AAD or PI protocol
Flow Cytometry

Note that I may want to: make shortcut sheet of calculations for CTL assay

Older Information