Eif1a
2015-01-13++ Eif1a
alignment of Eif1a to various other sequences here
"F:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis\22H10 aka 11\Eif1a alignment.ape"
Primers with Eif1a
REA TTTTTAAAAAACATTTAGATATTTCTCTAAGTCATTTTAATTC
REATr GAAATGTAGACACTGCAAGCAGG
FEA ggaaaggggcgtggcc
FEATr GTCACTACTCAGGAGGAGAC
FEATr2 (for a nested PCR)
cttccggagaaccaggcc
18 bp
54.7 Tm
REATr2 (for a nested PCR)
22 bp
54.7 Tm
CAGGAAATGGGAATACCTCAGG
FEATr+REATr (2667 bp) then FEATr2+REATr2 (2616 bp)