Dynazyme Protocol
2015-01-13azim58 - Dynazyme Protocol
extension time for dynazyme:
This is from the excel spreadsheet attached below. The amount of primers
reflect the amount for a primer concentration of 20uM. If the primer is a
different concentration other than 20uM, change the volumes or dilute
primer down to said concentration.
Protocol Here
"C:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\pcr_protocol\Dynazyme PCR.xls"
In our lab we use Dynazyme II DNA polymerase
I have some aliquots of dynazyme here: 7-2-13d1700 -20 C box
official manual here
http://www.thermoscientificbio.com/uploadedFiles/Resources/f503sl_dynazyme2_prodinfo_A4_TS_v1_low.pdf
and here
"F:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\dynazyme II dna polymerase product information.pdf"
see also Thermocycle Conditions portal 5-9-13
see also Dynazyme Colony PCR Protocol
see also comparison of primestar max with dynazyme 7-3-13