Designing Linkers 071110
2015-01-13azim58 - Designing Linkers 071110
A few slides about constructing scFv 7-12-10
"C:\kurt\storage\CIM Research Folder\DR\2013\1-28-13\A few slides about
constructing scFv 7-12-10.ppt"
Linker light sequence
TTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCG
Linker heavy sequence
CAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC
MHV_BACK_SfiI: CATATGGCCCAGCCGGCCA
MKV_FOR_NOTI:
GTTCTTGCGGCCGCCCGT
MLV_FOR_NOTI:
GTTCTGCGGCCGCCTTGG
Overlapping Linker Sequence
cagagccacctccgcctgaa
Light Chain Overlap with Linker
CGATCCGCCACCGCCAGA
Heavy Chain Overlap with Linker
GCCTCCACCTGAGGAGAC
Linker amp3 T sequence
CGATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC
MLV_BACK sequence
TTCCTGAGTCACAACAGCCTGCGATCCGCCACCGCCAGA
MHV_FOR1 sequence
GCCTCCACCTGCAGAGACAGTGACCAGAGT
original sequence
cgatccgccaccgccagagccacctccgcctgaaccgcctccacctgaggagac
Overlapping Linker annealing temp: 57.8
Light chain overlapping temp: 57.0
Heavy chain overlapping temp: 54.7
Overlapping linker primers for scFv Diagram
Overlapping Linker Primers for scFv.svg
code for finding temps of all mers for linker 7-13-10
"F:\kurt\storage\CIM Research Folder\DR\2013\6-21-13\wiki_download\code
for finding temps of all mers for linker 7-13-10"
Temps of all mers for linker 7-13-10
Temps of all mers for linker 7-13-10