Cut vector DNA 060612
2015-01-13azim58 - Cut vector DNA 060612
refer to Plan for Cloning Mouse scFv DNA 082411
Actually before I do any cloning, I need to modify the pComb3XSS vector
so that it just has 1 SfiI site and 1 NotI site in the appropriate
locations. In order to do this, I will cut with SpeI and NotI, and then
PCR amplify this product in a manner that excludes the NotI and SfiI
sites and also has sites of homology necessary for cloning the fragment
back into the vector.
Before modifying the vector, I just need to get more of it. I grew up a
culture and performed a miniprep.
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Item
pComb3XSS from miniprep stored at 10-3-11 box at -20 C
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