Chromium Release CTL Assay with Ova 8-17-12

2015-01-13

azim58 - Chromium Release CTL Assay with Ova 8-17-12


8-17-12 Chromium Release CTL Assay with Ova

In this experiment the target cells were resuspended to the wrong volumes
by accident, and therefore I did not have the E:T ratios I originally
planned. I also did not have enough targets to test the targets without
the peptide with the effectors at different ratios. I may repeat the
experiment.

Item: ova mouse sera 8-17-12 KW at CTL 6-13-11 KW -20 C

CTL Plate Map
L:\storage\CIM Research Folder\DR\2012\8-16-12\ctl plate map

Results
C:\kurt\storage\CIM Research Folder\DR\2012\8-18-12\Ova CTL

The results came out basically the way I expected them to. However, the
targets alone pulsed with peptide exhibited a lot of killing which I did
not expect.

Notes on paper used for plate layout 8-17-17
"L:\storage\CIM Research Folder\DR\2012\8-18-12\Ova CTL\Notes on paper
used for plate layout 8-17-12.pdf"