Cell lysate printing 8

2015-01-13

azim58 - Cell lysate printing 8


fairly fresh SMC1fs lysate diluted in plain non-induced pET32b lysate
printed onto nitrocellulose; intensity was detected well and morphology
looked good
found at 10-15-11

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate print 8 experiment 10-15-11 .pdf"

This print was of SMC1fs cell lysate diluted into plain non-induced
pET32b lysate printed onto a nitrocellulose slide. Although the spot
sizes are a little on the large side, there is not much smearing. The
intensity of the 1X and 0.5X spots are also clearly high enough to detect
over a negative spot.

Associated Files
"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Kurt_lysate_10-26-11_gal.gal"

"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 1.gps"

"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 1.gpr"

"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 1 data.xlsx"



"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 2.gps"

"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 2.gpr"

"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block 2 data.xlsx"



"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\SMC1fs Lysate Dilutions on Nitrocellulose 10-27-11.doc"


Pictures of Slides

Block 1
"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block_1.PNG"


Block 2
"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\block_2.PNG"

Graphs
Block 1
"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block_1_graph.PNG"


Block 2
"F:\kurt\storage\CIM Research Folder\DR\2013\4-27-13\wiki_download\cell
lysate printing 8\Block_2_graph.PNG"