Cell Lysate Printing 7

2015-01-13

azim58 - Cell Lysate Printing 7


"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Cell
Lysate Printing 7 Notes.pdf"

Experiment performed on 9-23-11

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate print 7 experiment 9-23-11 .pdf"


This print used this plate

morphology of cell lysate on nitrocellulose slides
"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2014\01-24-2014d0911\01-24-2014d0911\morphology_of_cell_lysate_on_nitrocellulose_slides.png"


Results: The nitrocellulose slides worked really well with good
morphology and little smearing. However, for some reason only the
positive control exhibited signal. The SMC1fs dilution series did not
show any signal. Perhaps this could be because this protein was produced
from bacteria transformed with a plasmid from colonies that had been on a
plate for several months.