Cell Lysate Print 4

2015-01-13

azim58 - Cell Lysate Print 4

Cell Lysate Test Print 4

(codelink and aminosilane condition test on 7-21-11)

This time denatured and non-denatured proteins were printed onto the
slides.

4 aminosilane slides
2 CodeLink slides
primary sera 1:500 rabbit anti-SMC1fs 27mer (non-purified)
aminosilane blocking: blocking buffer for both 30 m steps; CodeLink

blocking (CodeLink buffer 30m then incubation buffer 30m)

gpr files here
C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated
Records\2011\7-22-11

and here

S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\2011\7-22-11\Result Files

analyzed results here
"C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated
Records\2011\8-4-11\Analyzed Results\Analyzed Results.xlsx"

and here

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\Summary of Print Runs
9-5-13.svg"

Lab notebook notes

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate print 4 expreiment 7-21-11.pdf"

Basically, there was still a lot of smearing, and I was not able to
detect a 1/10 dilution of SMC1fs into PUC19 lysate above background.