Cell Lysate Print 3

2015-01-13

azim58 - Cell Lysate Print 3

experiment performed on 7-8-11

(codelink condition test)

used protocol CodeLink.hrp

CodeLink slides used and aminosilane slides tested
blocked 30 min with CodeLink ethanolamine blocking buffer (0.1 M Tris,

50 mM EtOH Amine Methol" (ethanolamine?), pH 9, blocked 30 min with BSA
containing buffer

4 slides 5 nM primary (purified rabbit anti-h-SMC1fs 17 mer)
6 slides 1:500 sera (rabbit anti-h-SMC1fs)
5 nM secondary (Bethyl biotinylated goat anti-Rabbit IgG)
5 nM tertiary (streptavidin AF647)

results here
F:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated
Records\2011\7-12-11\Cell Lysate Array 3 Results

and here

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\Summary of Print Runs
9-5-13.svg"

lab notebook notes corresponding to this experiment

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate 3 experiment 7-8-11.pdf"

These codelink slides still appeared quite smeared.