Cell Lysate Print 13
2015-01-13azim58 - Cell Lysate Print 13
Cell Lysate Print 13 (Grace BioLabs Nitrocellulose Slides 16 hr room
temperature incubation; one with Super G blocking buffer one without)
Slides ran around 1-20-12
Spacing of Spots when printed: 500 um
Primary: Balb/Nat SMC1 27 mer
Secondary: goat anti mouse
Data Location:
L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical
Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA
Library\Printing Library onto array\Cell Lysate Test Prints\BSA Super G
Test 1-21-12
2-7-12
I was going to align and analyze these arrays, but after looking at the
images of the slides, I see that the spots were very faint and quite
smeared. Did the overnight condition cause these undesirable results?
Johnny and I will print 4 more slides for a test.
1 slide BSA primary incubation 1 hr
1 slide Super G primary incubation 1 hr
1 slide BSA primary incubation 16 hr
1 slide Super G primary incubation 16 hr