Cell Lysate Print 12

2015-01-13

azim58 - Cell Lysate Print 12


Cell Lysate with Super G Blocking Buffer Summary
Grace BioLabs Nitrocellulose Slides with Super G Blocking Buffer 2 hr
primary at 37C Summary
found at 12-19-11

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate print 12 experiment 12-19-11 .pdf"



The Super G Blocking Buffer appears to decrease all intensities
considerably. However, the ability to distinguish between different
lysate dilutions and the ability to detect lower dilutions seems to
increase. The error bars of replicate lysate spots also seems quite small.

Location of Data:
S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\2011\12-18-11
and here
L:\storage\CIM Research Folder\DR\2011\12-18-11

Sera: BALB Nat anti-mSMC1 27 mer Low anti-t Hap2 + Rbrwh Pooled 10/15/11
Some Details of Experiment 12-19-11

Two Dilutions of primary sera were tested on the two different blocks on
this slide (upper chamber and lower chamber)

Top Block (1:20 primary dilution)




Bottom Block (1:500 primary dilution)
"F:\kurt\storage\CIM Research
Folder\DR\2013\5-27-13\wiki_download\Bottom_Block.png"
"F:\kurt\storage\CIM Research
Folder\DR\2013\5-27-13\wiki_download\Bottome Block Graph 2 12-18-11.png"


Brain Cancer Super G Comparison