Cell Lysate Plate 9-21-11
2015-01-13azim58 - Cell Lysate Plate 9-21-11
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Lysate Plate 9-21-11.one"
Cell Lysate Plate 9-21-11
Wednesday, September 21, 2011
7:10 PM
Denatured proteins: 1% SDS 1 hr 60 C (50 uL original sample)
Plate Layout
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
A (non-denatured)
1X
0.5X
0.1X
0.01X
0.001X
0.0001X
0
GST
GST-SMC1fs
Old 1X from 8-10-11
1X
0.5X
0.1X
0.01X
0.001X
0.0001X
0
GST
GST-SMC1fs
Old 1X from 8-10-11
B
C (denatured)
1X
0.5X
0.1X
0.01X
0.001X
0.0001X
0
GST
GST-SMC1fs
Old 1X from 8-10-11
1X
0.5X
0.1X
0.01X
0.001X
0.0001X
0
GST
GST-SMC1fs
Old 1X from 8-10-11
EDTA was added to samples (final concentration: 1 mM) to prevent
bacterial growth.
GST was obtained from Shen's shelf: GST protein from pGEX2T in PBS-2
1/28/11
SMC1 protein was obtained from Shen's shelf
hSMC1-27mer 1 mg/mL 6/28/11
I diluted this protein 5 fold (40 uL into 200 uL 1X PBS) for 0.2 mg/mL
Nanodrop Concentrations:
Sample
Concentration (ng/mL)
260/280
pGEX-SMC1fs Induced
11.54
0.82
pGEX-SMC1fs Not Induced
13.46
0.73
pET32 TEV
8.58
0.55
pET32 TEV Not Induced
11.25
0.72