Basic plan for random hexamer library construction 1-24-13
2014-08-29azim58 - Basic plan for random hexamer library construction 1-24-13
I will construct the library using four different methods and then
compare the complexity of each method.
for primers used see cDNA Library Random Hexamer Plan 12-12-12
Synthesize 1st strand cDNA from poly A purified RNA using reverse
transcriptase in In-Fusion SMARTer Directional cDNA Library Construction
Kit -> 2nd strand synthesis with PrimeStar
Synthesize 1st strand cDNA from poly A purified RNA using reverse
transcriptase in In-Fusion SMARTer Directional cDNA Library Construction
Kit -> 2nd strand synthesis with dynazyme
Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript
reverse transcriptase -> 2nd strand synthesis with PrimeStar
Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript
reverse transcriptase -> 2nd strand synthesis with dynazyme