Background Info for Immunosignature to Antigen Work
2014-08-29azim58 - Background Info for Immunosignature to Antigen Work
My DoD Breast Cancer Predoctoral Traineeship application has some good
background information about my project.
"F:\kurt\storage\CIM Research
Folder\DR\2013\6-16-13\wiki_download\Identification of Breast Cancer
Antigens through Immunosignatures.doc"
"F:\kurt\storage\CIM Research
Folder\DR\2013\6-16-13\wiki_download\References Cited.odt"
Here's a basic outline of the paper
- Career Goals
- Training
o Peter Lee, Kathryn Sykes
- Research Project
- description of antigen discovery platform
- 2 reagents
@ antibody-carrying sera
@ random sequence 20 mer peptides
- SMC1fs positive control
- eventual cancer vaccine
o Background
- tumor associated antigen repressed mammary tumors in rats 9
- whole cell tumor lysate was immunogenic 10
- self tumor mixtures in clinical trials 11
- more antigens would be good
- more specific antigens needed
- tumor associated antigen examples: HER2/neu, MUC1, MAGE
- Specific Antigens: frameshifts, chromosomal rearrangements,
aberrant protein modifications
- example of tumor specific antigen from lymphoma group 13
- learn from immune system (from antibodies)
- random peptide array can distinguish diseases
- need to discover original antigen
- SMC1fs positive control
- SMC1fs found more in cancer than normal cells
- plan for SMC1fs sera
- phage display technology
@ small antibodies
@ unlimited amount of phage
- compare sera and library
- conclusion
o Methods
- Identification of breast cancer specific immunosignature
@ format of slides
@ basic procedure
@ process for determining informative peptides
@ different random arrays could be used
- Probing biological samples
@ random peptide array helps purify antibodies
@ probe biological space
# Western blot
# cDNA library
- Phage Library Approach
@ Process for making phage library
- Discovering human antigens
o Relevance
Cancer vaccine references
- Vaccination with soluble low-molecular weight tumor-associated
o I wasn't able to get an electronic copy of this paper online
normally, but I did get it from the ILLIAD ASU service
- "F:\kurt\storage\CIM Research
Folder\DR\2013\6-16-13\wiki_download\Vaccination with soluble
low-molecular weight tumour-associated proteins suppresses
Chemically-induced Mammary Tumorigenesis in Rats.pdf"
- Immunotherapeutic potential of whole tumour cells
xjvyrv/fulltext.pdf
- Cancer vaccines: between the idea and the reality
i1150.pdf
Shen also has some data to show that a cancer vaccine is effective.
"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\cancer
vaccine protection .ppt"
Questions
Have I found a paper that shows that scFv bind the same antigen as the
original antibody? I think I've read this before, but now I don't know
where it is.