Alignment with whole plasmid 070512
2014-08-29azim58 - Alignment with whole plasmid 070512
reverse_primer ------------------------------------------------------------
pcomb AGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG 60
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTA 120
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG 180
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AATTGTGAGCGGATAACAATTGAATTCAGGAGGAATTTAAAATGAAAAAGACAGCTATCG 240
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CGATTGCAGTGGCACTGGCTGGTTTCGCTACCGTGGCCCAGGCGGCCGAGCTCGCCATGG 300
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTGGTTGGGCAGCGAGTAATAACAATCCAGCGGCTGCCGTAGGCAATAGGTATTTCATTA 360
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGACTGTCTCCTTGGCGACTAGCTAGTTTAGAATTCGTAATCATGGTCATAGCTGTTTCC 420
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTG 480
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC 540
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CGCTTTCCAGTCGGGAAACCTGTCGTGTTACTAATGATGGTGATGGTGATGGCTAGTTTT 600
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GTCACAAGATTTGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCAC 660
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCTGAG 720
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC 780
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCCTGAGTTCCACGACACCGTCGCCGGTTCGGGGAAGTAGTCCTTGACCAGGCAGCCCAG 840
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCC 900
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTACCAGCAGAAACCTGGCCAGGCTCCCA 960
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GGCTCCTCATCTATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCA 1020
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAG 1080
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGTACTACTGTCAGCAGTATGGTGGCTCACCGTGGTTCGGCCAAGGGACCAAGGTGGAAC 1140
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA 1200
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAG 1260
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC 1320
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT 1380
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb ACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTTGCCCGTCA 1440
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CAAAGAGCTTCAACAGGGGAGAGTGTTAGTTCTAGATAATTAATTAGGAGGAATTTAAAA 1500
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGAAATACCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCTGCCCAACCAGCCA 1560
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGGCCGAGGTGCAGCTGCTCGAGATGAGCGATAAAATTATTCACCTGACTGACGACAGTT 1620
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGT 1680
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCA 1740
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCA 1800
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGG 1860
reverse ------------------------------------------------------------
forward ---------------------------------------------------NNNNNNNNG 9
reverse_primer ------------------------------------------------------------
pcomb GTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTACCCGTACG 1920
reverse ------------------------------------------------------------
forward NNGNNNTGNCTAN-GGNCAGTTGAA-GAGTTCCTCGACGCTAACCTGGCGTACCCGTACG 67
reverse_primer ------------------------------------------------------------
pcomb ACGTTCCGGACTACGGTTCTACTAG-TGGCCAG------GCCGGCCAGCACCATCACCAT 1973
reverse ---------------------------------------NNTTNNTAGCACCNTCACCAT 21
forward ACGTTCCGGACTACGGTTCTACTAGGCGGCCGCTTTTTTTTTTTTTAGCACCATCACCAT 127
reverse_primer ------------------------------------------------------------
pcomb CACCATGGC-GCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 2032
reverse CANCATGGNCGCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 81
forward CACCATGGC-GCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 186
reverse_primer ------------------------------------------------------------
pcomb TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 2092
reverse TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 141
forward TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 246
reverse_primer ------------------------------------------------------------
pcomb TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 2152
reverse TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 201
forward TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 306
reverse_primer ------------------------------------------------------------
pcomb TGCCGATGAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 2212
reverse TGCCGATGAAAACGTGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 261
forward TGCCGATGAAAACGTGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 366
reverse_primer ------------------------------------------------------------
pcomb TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 2272
reverse TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 321
forward TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 426
reverse_primer ------------------------------------------------------------
pcomb TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 2332
reverse TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 381
forward TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 486
reverse_primer ------------------------------------------------------------
pcomb TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 2392
reverse TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 441
forward TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 546
reverse_primer ------------------------------------------------------------
pcomb TCGCCCTTTTGTCTTTAGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 2452
reverse TCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 501
forward TCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 606
reverse_primer ------------------------------------------------------------
pcomb AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 2512
reverse AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 561
forward AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 666
reverse_primer --------TTGCTAACATACTGCGTAATAAGGAG-------------------------- 26
pcomb TTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAAGCTAGCTAATTAATTTAAGC 2572
reverse TTCTACGTTTGCTAACATACTGCGTAATAAGGAGGCC-------GCAGATCTGCTCT--C 612
forward TTCTACGTTTGCTAACATACTGCGTAATAAGGAGGCC-------GCAGATCTGCTCT--C 717
**************************
reverse_primer -GCCGCAGATCTGGGAAATTG--------------------------------------- 46
pcomb GGCCGCAGATCTGGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTT 2632
reverse TGAGGAGGATCTGGGAAATNGTAAGCGTTAATATTTTNTTAAAATNCGCNNNNAANNNNN 672
forward TGANGAGGATCTGGGAAATTGTAAGCGTTAATATTTTGNTAAAATTCGCGTTAAATTTTT 777
* * ************ *
reverse_primer ------------------------------------------------------------
pcomb GTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAA 2692
reverse NNNNNN------------------------------------------------------ 678
forward GTTAANTCAGCTCATTTTTTAACCAATNNNCCGAAATCGNNAAA-TCCCTTATAAATCAA 836
reverse_primer ------------------------------------------------------------
pcomb AAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAA 2752
reverse ------------------------------------------------------------
forward A-GAATANACCGANATANGGTTNAGTGTTGTNCN-GTTTGNNACNN-ANNCCACTATNNN 893
reverse_primer ------------------------------------------------------------
pcomb AGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTAC 2812
reverse ------------------------------------------------------------
forward N-AANGTGGANTCCAANNGNNNNNNNNNNANN-CNGTCTATCNNGNNNANGGCNNNNAN- 950
reverse_primer ------------------------------------------------------------
pcomb GTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGA 2872
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb ACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAA 2932
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGC 2992
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCA 3052
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATA 3112
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGA 3172
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTC 3232
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTG 3292
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC 3352
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTAT 3412
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT 3472
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT 3532
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGA 3592
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCC 3652
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA 3712
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAG 3772
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGC 3832
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGT 3892
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCT 3952
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb ACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG 4012
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTG 4072
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb ATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCA 4132
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGA 4192
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA 4252
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGA 4312
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGT 4372
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGT 4432
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAT 4492
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT 4552
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCA 4612
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAG 4672
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTC 4732
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb GCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGA 4792
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb AAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACA 4852
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb TGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAG 4912
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer ------------------------------------------------------------
pcomb CTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGG 4972
reverse ------------------------------------------------------------
forward ------------------------------------------------------------
reverse_primer -
pcomb A 4973
reverse -
forward -