7-AAD or PI protocol

2014-08-29

azim58 - 7-AAD or PI protocol


7-AAD or PI protocol

Before starting: Dilute 10X binding buffer to 1X using distilled water
(1mL 10X binding buffer + 9mL dH20)

  1. Wash cells once in PBS
  2. Wash cells once in 1X binding buffer (prepared above)
  3. Resuspend cells in 1X binding buffer at 1-5x10^6/mL
  4. Add 5uL of PI staining solution OR 7-AAD staining solution.
  5. Analyze by flow cytometry within 4 hours, storing at 2-8C in the dark

Note that to run through our flow cytometer, should add 1X PBS right
before analysis so that the final volume is 1mL (as preferred by the
cytometer)



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