3-25-13 sequence investigation

2014-08-29

azim58 - 3-25-13 sequence investigation

Mara sent our pcomb plasmids for sequencing with SP6, SP7, and SP8. None
of the sequences came back well.

<sequencing results> e-mail
https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13d4b4670179
cc87

https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13d9a0e7f270
1231
</sequencing results>

sequencher file of sequencing results
"C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\pcomb
sequencing results.SPF"

raw pcomb sequencing files
"C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\raw pcomb
sequencing files"

Should the primers have aligned with the expected pcomb3xssv3 sequence?
Yes they should have. Here is a sequencher file showing 100% alignment.
"C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\alignment of 6
7 and 8 with expected pcomb3xssv3.SPF"