11-5-12 Correct deletion of stop codon
2014-08-29azim58 - 11-5-12 Correct deletion of stop codon
11-5-12 Correct Deletion of Stop Codon
The stop codon of the pIII protein was deleted while I was making
changes, and this needs to be corrected.
see
C:\kurt\storage\CIM Research Folder\DR\2012\11-5-12\pcomb\readd stop codon
primers ordered
FP1
RP1
FP2
RP2
LP1
LP2
These primers were received and have a date of 11-15-12 store in 4 C Kurt
11-16-12
size of linearized pComb MN should be 4899
size of pComb fragment to correct deletion of stop codon should be 139 bp
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fragment to in-fuse into vector is stored at -20
(these are gel extracted fragments)
fragment UV 11-16-12
fragment no UV 11-16-12
fragment min UV 11-16-12
The PCR products necessary to create these gel extracted fragments are
also stored at -20 C
PCR Product 11-16-12
See notebook with corresponding date for details