10K random peptide array

2014-08-29

azim58 - 10K random peptide array
CIM10k portal

density of spot on array

Peptide Concentration: 1 mg/mL

o 1 mg/mL*1g/1000mg*1mol/(110*20)g*1000mmol/1mol = 4.55*10^(-4)
mmol/mL or 0.455 umol/mL
o Corresponding density per surface area: 0.455
umol/mL*0.3mL/(Pi*(70um)^2) = 8.87 mol/m^2

Spot volume: 300 uL
Spot size is about 140 um in diameter
see 6-29-12 e-mail from Zbig for original source
average molecular weight of amino acid (110 Daltons)

(http://www.genscript.com/conversion.html)

Phil said that on the aminosilane slides the peptides are about 1 nm

apart so there would be about 4 peptides/(1 nm^2)

versions of cim10k arrays
10kv1
GSC on C terminal (right side of rest of sequence since sequences are listed N to C)

10kv2
GSC on N terminal (left side of rest of sequence since sequences are listed N to C)

10kv3
used D amino acids (written as lower case letters by our lab)

some summary notes from Bart
https://mail.google.com/mail/u/0/?ui=2&shva=1#search/Bart/143de90deac1d86c



CIM10Kv1            20 mer, 17 random C term GSC linker.  All aa except Cys in random positions





CIM10Kv2            20 mer, 17 random N term CSG linker.  All aa except Cys in random positions





CIM10Kv3            20 mer, 17 random N term CSG linker.  All aa except Cys in random positions, contains some d amino acids (lower case letters) and some with C term beta-alanine