1-14-13 linearize plasmid

2014-08-29

azim58 - 1-14-13 linearize plasmid


1-14-13

Plan to linearize pComb MN vector again.

35 s extension with 1 ng worked the best before (see 11-27-12 of notebook)
primers are LP1 and LP2
annealing temperature of 56 or 58 seemed to work the best before (see
11-28-12 of notebook)

size of linearized pComb MN should be 4899