tumor antigens nitrocellulose array search 4-25-12

2014-08-29

azim58 - tumor antigens nitrocellulose array search 4-25-12

tumor antigens nitrocellulose array

Evaluation of humoral response to tumor antigens using recombinant
expression-based serological mini-arrays (SMARTA)
Moscow State University
SMARTA (serological mini-arrays of recombinant tumor antigens)
multianalyte microspot immunoassays
they had about 7mm spots with 10^3 to 10^6 pfu of phage per spot

Diagnostic Markers of Ovarian Cancer by High-Throughput Antigen Cloning
and Detection on Arrays
by Barbara Ann Karmanos Cancer Institute in Detroit Michigan
they use the immune system as a biosensor
they biopan an ovarian cancer phage display library using sera from a
cancer patient
they had protein macroarrays containing 480 selected antigen clones

Identification of tumour antigens by serological analysis of cDNA
expression cloning
by The Nottingham Trent University in the UK
quote: To date, over 2,000 tumour antigens have been identified from a
variety of malignancies using SEREX.
they talk about antigen-specific cancer vaccines
SEREX was developed by Pfreundschuh and his colleagues Sahin and Tu¨ reci
SEREX generally uses lambda phage expression vectors
More than 2,000 antigens have been listed in the SEREX database [29].
Perhaps the SEREX database is now part of the Cancer Immunome Database?
http://ludwig-sun5.unil.ch/CancerImmunomeDB/
SEREX has identified mutated gene products as well as splice variants
cancer/testis (CT) antigens.
note: Since CT antigens are immunogenic and highly restricted to tumours,
their discovery has led directly to the development of antigen-specific
cancer vaccines
this is an interesteing antigen to me: quote: Since CT antigens are
immunogenic and highly restricted to tumours, their discovery has led
directly to the development of antigen-specific cancer vaccines expressed
in cancer tissues compared with matched normal tissues
quote: After screening at least half a million clones per library,
multiple reactive clones were identified in each library.
quote: We have developed an array technique which can beused to rapidly
analyse humoral response of multipleSEREX antigens using allogeneic sera.
One microlitre ofmonoclonal positive phages (200–300 pfu/ll) is
spottedside by side with nonrecombinant phages directly ontothe agar
plate premixed with host cells. Following anovernight incubation,
‘‘phage array’’ is transfered ontonitrocellulose membrane and
screened with preabsorbedand diluted allogeneic sera [19].
they do pre-absorption of their sera with E.coli and/or phage lysate
they state it would be better to develop eukaryotic expression systems
for SEREX

Microarrays of tumor cell derived proteins uncover a distinct pattern of
prostate cancer serum immunoreactivity
by Van Andel Research Institute in Grand Rapids Michigan, Johns Hopkins
in Maryland and NovoDynamics in Michigan
they use prostate cancer sera against 2D liquid chromatography purified
prostate cancer proteins (1760 fractions)
with selected proteins, they could classify samples with 98% accuracy
they performed heirarchical clustering of their data
with their method they cannot identify the proteins and they just refer
to the high antibody binders by their fraciton id

Tumor-associated Antigen Arrays for the Serological Diagnosis of Cancer*
from the Scripps research Institute
they specifically choose the tumor associated antigens beforehand
they have a nice table of candidate TAAs recognized by autoantibodies
from prostate cancer patients

I still need to check this paper
Serologic analysis of ovarian tumor antigens reveals a bias toward
antigens encoded on 17q

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