tail suspension test portal 11-25-2014d2227

2015-04-30

hold mouse by tail and measure movement

-tail suspension test
--http://en.wikipedia.org/wiki/Tail_suspension_test
-The tail suspension test paper (Can, Adem et al. “The Tail Suspension Test.” Journal of Visualized Experiments : JoVE 59 (2012): 3769. PMC. Web. 30 Nov. 2014.)
--test usually for 6 min
--http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353516/
---there is a video at that link too
--measure time mice spends struggling
--they use tape (17 cm long fragments) to suspend the mouse.  They attach the tail to the middle of the 2 cm portion at the end of the tape.
--they record the mouse
--compounds with antidepressant properties increase the amount of time mice spend struggling.  This is reflected by shorter periods of immobility
--multiple mice can be suspended at the same time and recorded
--they do an acclamation period to the room for 1 hr
--they use plastic cylinders around tail to prevent the mouse from climbing its own tail.  Their cylinders were 1 cm in diameter, 4 cm long, and 1.5 g.
--typically at the end they graph immobility time
--they don't count the movement if it only involves the front legs

-[cni_automated tail suspension video analysis with imageJ 04-30-2015d0957]

online stopwatch controlled by enter key
 http://online-stopwatch.chronme.com/
 
 
 
Some items
-[cni_investigated possible analysis methods with Diego 04-21-2015d1624]

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See also
-[how to open an mp4 or other movie file in imagej 04-21-2015d0029]

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