stem cell comments from John 1-24-13

2014-08-29

azim58 - stem cell comments from John 1-24-13

original e-mail thread here
https://mail.google.com/mail/u/0/?ui=2&amp;shva=1#label/Career/13c6a04ac1c0
d8ae

text from John

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Yeah that's what I thought. This is a unipotent-lineage depletion set. So
it would give you a population similar to CD34 positive selection. These
cells will be 99%+ multipotent progenitors. (i.e. a cell with limited
replicative potential that does not self-renew). Progenitors do not
express the carcinoembryonic self-renewal associated genes that
originally inspired your hypothesis. If you'd get rid of the progenitors
and looked at only "true" (i.e. self-renewing, long-term repopulating)
hematopoietic stem cells, you should see a big increase in signal if your
hypotheses are true, and no increase in signal if they are wrong.

There are marker-sets that give you pure HSC in mice, but no easy
magnetic things, due to the complicated marker combinations. So you'd
need to use flow sorting (Meaning every single cell purified in this way
will be sufficient for multilineage repopulation of an irradiated scid
mouse). Unfortunately, no marker sets are known that enrich human HSC to
more than 10%.

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