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2014-08-29

azim58 - search 4-26-12 tumor antigens nitrocellulose array SEROLOGIC ANALYSIS OF OVARIAN TUMOR ANTIGENS REVEALS A BIAS TOWARD ANTIGENS ENCODED ON 17q by Institute for Systems Biology in Seattle with other Seattle groups (2003) they used sera from 25 ovarian cancer patients to screen 3 independent cDNA libraries. They found 9 antigens. 4 of these antigens were in the 17q chromosome region. Their cDNA library was in the form of phage sera was precleared of E. coli-specific antibodies using an E. coli affinity resin 5Prime-3Prime E-mail conversation about this 5-4-12 plaques were originally screened in a low throughput way. They did use an array-based secondary screening method though. However, this array was a 6X8 array. They made it so they could reproducibly keep screening a lot of different sera. Antibody detection using tumor-associated antigen mini-array in immunodiagnosing human hepatocellular carcinoma by Scripps Research Institute and many other universities in 2006 they just screen sera against a panel of 8 antigens. They just performed ELISA, and used it mostly for diagnoses. Serological identification of embryonic neural proteins as highly immunogenic tumor antigens in small cell lung cancer Ludwig Institute for Cancer Research in New York SEREX against small cell lung cancer cell lines. They found embryonic genes in the tumors their tumor cDNA was expressed on phage Serological and proteomic evaluation of antibody responses in the identification of tumor antigens in renal cell carcinoma by St. James's University Hospital in UK in 2003 they use a SPEAR approach which combines 2D polyacrylamide gel electrophoresis of tumor samples with immunoblotting and protein identification by mass spectrometry original SEREX paper in 1995 Human neoplasms elicit multiple specific immune responses in the autologous host by Pfreundschuh in University of Searland Medical School in Homburg Germany they expressed tumor cDNA libraries in phage that infected E. coli and screened with autologous patient serum. They discovered at least 4 antigens this way there was much more antibody reactivity than they expected they screened their library in a low-throughput nitrocellulose membrane manner Cellular and humoral immune responses to a human pancreatic cancer antigen, coactosin-like protein, originally defined by the SEREX method by Kumamoto University in Japan quote: Cellular and humoral immune responses to a human pancreatic cancer antigen, coactosin-like protein, originally defined by the SEREX method they looked for pancreatic cancer antigens they focus on coactosin-like protein CLP they did their SEREX using the standard plate method Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX by Dana Farber Cancer Institute at Harvard and Benjamin Franklin Universiyt in Berlin Germany they cloned the tumor cDNA library into phage they use nitrocellulose membranes new search: high throughput serex Highthroughput proteomic strategies for identifying tumour-associated antigens mentiones SERPA (2D electropheresis followed by mass spec) Identification of prostate cancer antigens by automated high-throughput filter immunoscreening their method has 18,000 clones they use phage for tumor cDNA library
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