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poly dT primer portal 2-1-13

2014-08-29

azim58 - poly dT primer portal 2-1-13 The purpose of a poly dT primer would be to amplify poly A mRNA. One might want to make 1st strand cDNA from RNA, and then 2nd strand cDNA from 1st strand cDNA. Here are some primers I will order. dT1 poly T primer for in-fusion with vector in directional SMARTer In-Fusion kit 5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTTVN–3’ dT2 2nd strand synthesis primer for in-fusion with vector in directional SMARTer In-Fusion kit 5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTT–3’ dT3 poly T primer (this could be used for 1st and 2nd strand synthesis 5'-TTTTTTTTTTTTTTTTTTTTVN–3’ "C:\kurt\storage\CIM Research Folder\DR\2013\2-1-13\poly dT primer\Primer order form Kurt 2-1-13.xls"
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