pIII protein of M13 phage

2014-08-29

azim58 - pIII protein of M13 phage

* There are typically 3-5 copies of pIII at one end of the phage
  whereas there are many pVIII proteins all around the virus
* The pIII protein appears to bind directly to phage. Note that I am
  not completely sure if phi 29 phage works the same as m13 phage in this
  regard.
  o http://www.pnas.org/content/77/11/6425.full.pdf
    - In this paper, we show that protein p3 is linked to the 5'
      termini of 029 DNA through a phosphoester bond between serine and
      5'-dAMP. A
      preliminary account of some of these findings has been pre-sented

o 1. Protein p3 is linked to the DNA of phage 029 through a
    phosphoester bond between serine and 5'-dAMP (initiation of
    replication/Bacillus subtilis/nucleotidyl-peptide/acid and alkali
    hydrolysis). JosE M. HERMOSO AND MARGARITA SALAS Centro de Biologla
    Molecular (CSIGUAM), Universidad Autonoma, Canto Blanco, Madrid-34,
    Spain
  o 
    2. Gene 1:25-36 Sambrook, J. & Russell, D. (2001) Molecular Cloning:
    A Laboratory Manual, Third Edition, (Cold Spring Harbor Laboratory
    Press, Cold Spring Harbor, NY).

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