pComb3XSS Vector

2014-08-29

azim58 - pComb3XSS Vector

Size: 4973
Sequence:
http://www.scripps.edu/mb/barbas/content/pcomb_images/pcomb_images_files/pC
omb_Text_Files/pComb3XSS.txt
Original Map from Scripps:
http://www.scripps.edu/mb/barbas/content/pcomb_images/pcomb_images_files/pC
omb_Maps/pComb3X_Maps.pdf

NotI = GCGGCCGC
SpeI = ACTAGT
SfiI = GGCCNNNNNGGCC

NotI = 2570bp
SpeI = 1940bp
SfiI = 274bp, and 1946bp (GGCCCAGGCGGCC, GGCCAGGCCGGCC)

Fragment sizes when cutting with SpeI and NotI
630bp
4343bp
Sequence of Fragment Cut by SpeI and 060612

some potential annotations

* lac prom 145-174
  amp 3174-4034
  f1 ori 2720-3026
  pBR322 ori 4190-4809
  Light Chain Stuffer from SacI to XbaI 293-1473
  Heavy Chain Stuffer from XhoI to SpeI 1580-1940
  geneIII from amber stop codon to NheI 2016-2552
  SacI 293 (GAGCTC)
  XbaI 1473
  XhoI 1580
  SpeI (ACTAGT) 1940
  NheI (GCTAGC) 2552
  amber stop codon (TAG) 2016
  SfiI_1 274 (GGCCCAGGCGGCC)
  SfiI_2 1946 (GGCCAGGCCGGCC)
  NotI 2570

Plan for modifying pComb3XSS vector 061312

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Note that a new vector was made from this vector: pComb3XSSv2
pComb3XSSv3

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Request of pComb3XSS vector
e-mails regarding request of pComb3XSS vector 3-25-12
pComb3xSS ships June 4th 2012!
Received vector from Scripps 6-5-12
pComb3XSS 10 uL of 10 ng/uL at -20 C

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