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fluors written on side of instrument
2013-12-01
azim58 - fluors written on side of instrument F1 FITC F2 PE F3 ECD F4 PC5 F5 PC7 Fluor Configurations What are the emission wavelengths for these 5 dyes? some good resources: http://rhlccflow.facilities.northwestern.edu/files/2011/09/Fluorochrome.pdf http://dbc.bio.uci.edu/OBCresources/fluorophores.pdf F1 FITC: excitation/emission 468-509/504-541 F2 PE (Phycoerythrin): excitation/emission 486-580/568-590 F3 ECD (PE-Texas Red): excitation/emission 486-575/610-635 F4 PC5 (PE-Cy5): excitation/emission 486-575/660-680 F5 PC7 (PE-cY7): excitation/emission 486-575/750-810 What about some other dyes? 7-AAD: excitation/emission 488/655 PI (propidium iodide): excitation/emission 536/617 APC: excitation/emission 600-640/660
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