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electroelution protocol

2013-12-01

azim58 - electroelution protocol Electroelution protocol see Electroelution with Elutrap referred to protocol here http://humgen.wustl.edu/hdk_lab_manual/rdm/rdm9.html Note: minimize exposure to UV (use prep (long form) of UV to cut out the DNA Note: expected yield is 50-75% -Cut a piece of dialysis tubing approximately 3 cm longer than the gel slice and clip one end. Gently push gel slice into open end and down to the clip. Add some buffer -Place gel slices parallel to the electrodes and fill the electrophoresis box with buffer (again, same as original gel) until all tubing is submerged. -Electroelute at 80-100 volts for 2-5 hours, longer for large fragments. The movement of the DNA can be monitored with a hand held UV source (long-wavelength). -DNA may stick to dialysis tubing after current flow. Remove the gel piece from the dialysis tubing. Reverse the electrodes and run for 15 s to 2 min to dislodge the DNA from the membrane surface.
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