choosing laser power and gain

2013-12-01

azim58 - choosing laser power and gain

5 things that affect the quality of images
http://swehsc.pharmacy.arizona.edu/exppath/core/equip/handouts/Zeiss510_-_5
_things.pdf

higher gain amplifies the signal but also increases noise
laserpower that is too high can photobleach the sample and possibly
reduce optical resolution

I guess I would want to scan the slide so that I obtain the greatest
dynamic range without a lot of spots maxing out, and the least noise
I could probably determine good settings for a certain type of slide
initially by starting with the highest laser power, and then working my
way down with the gain and laser power until the slide is no longer
saturated and is not losing signal to noise ratio.

How to Set the Correct Gain in the LS Scanner
http://www.tecan.com/platform/content/element/2508/Tecan_TN_LSX_GainSetting
s_394563_V1.0.pdf

The most common definition of sensitivity relates to signal/noise ratios.
sensitivity = (Signal-Background)/Noise

signal = average signal of a spot
background = average signal of background
noise = standard deviation of background

azim58wiki: