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amplify specific transcripts from RNA 4-11-13

2013-12-01

azim58 - amplify specific transcripts from RNA 4-11-13 I plan to try to amplify transcripts with RRS8+FRS8 and REATr+FEATr starting from RNA again. With PrimeSTAR Max perhaps a reasonable procedure would be to add 1 uL of 2 uM each primer and do 30 cycles. Then add 1 uL 10 uM each primer, 5 uL H20, 5 uL PrimeSTAR Max Premix, and perform 10 more cycles. see
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