Tumor Library Nitrocellulose Print 5-31-12

2015-01-13

azim58 - Tumor Library Nitrocellulose Print 5-31-12

Print onto Grace Biolabs Nitrocellulose Slides (dimensions are on that
page)

The print will start about 5 mm down and 2.9 mm over. The pad starts
about 3 mm down and 2.5 mm over.
The print will cover about 53 mm down and 7.4 mm across. The pad covers
about 60 mm down and 20 mm over.
So everything should be able to fit.

Pin Operation

* Dip into peptide
* pretap 5X on one slide
* print 1 spot on 4 slides
* repeat pin cleaning 3X
  o sonicate 30 s
  o wash in 50% acetonitrile 10 s
  o dry 5 s

* Sonicate 30 s
* wash 30 s
* dry 40 s

Landing Light Concentration: 1:100
Landing Light Identity: a biotinylated peptide. Therefore, in order to
detect the landing lights, it is necessary to use streptavidin
AlexaFluor. If direct labeled secondary is used, the landing lights will
not be visible unless a small of amount (such as 0.1 nM) of streptavidin
AlexaFluor is added as well.

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Johnny will do a practice print of this on Monday.
He will do the actual print on Tuesday (6-5-12)

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