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Transformation to determine efficiency and complexity 10-25-12

2015-01-13

azim58 - Transformation to determine efficiency and complexity 10-25-12 Transformation to determine efficiency and complexity 10-25-12 Finished ethanol precipitating the in-fusion reaction started by Mara. Here are the sample IDs 10-24-12 MM Positive 18.1 ng/uL 1.61 260/280 Negative 20.8 ng/uL 1.85 260/280 Control A 30.4 ng/uL 1.68 260/280 Tumor A 40.0 ng/uL 1.67 260/280 Samples stored at Human tumor cDNA 8-3-12 -20 C 0.25 ng/(2 uL) = 0.125 ng/uL solutions were made from these to do a transformation in which there would be about 500 colonies on a plate spread with 50 uL solution if the transformation efficiency were 4e6 cfu/ug.
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