Titration of Secondary Antibody 4-19-12

2015-01-13

azim58 - Titration of Secondary Antibody 4-19-12

experiment performed on 4-17-12

Image Files can be found here
L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical
Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA
Library\Application of sera onto array\sec titr 4-18-12
or
https://imtiewhl.livedrive.com/files/4424
or
S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor
cDNA Library\Application of sera onto array\sec titr 4-18-12

Analysis

These results did not turn out very well. The correlation coefficient
between samples is not very good, and the 0.1 nM secondary sample has a
lower intensity than the 0.01 nM sample. Fortunately, these samples do
have a lower intensity than the 5 nM samples. I think the cause of these
unexpected results was that the single chamber frames had very old rubber
gaskets which do not seal well and cause leaking to occur. This leaking
resulted in several slides breaking when the chamber was opened, and
streaks on the slides also made it so that many spots had to be marked as
bad rather than analyzed.

Secondary Titration Summary 4-30-12
"S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor
cDNA Library\Application of sera onto array\sec titr
4-18-12\analysis\Secondary Titration Summary.docx"

Secondary Titration Intensities

Correlations
2 0.01 nM Samples
0.0802

3 5 nM Samples
0.0.323 r^2 of 5_nM_2 with 5_nM_3
0.0596 r^2 of 5_nM_2 with 5_nM_1

Sample Intensities

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