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Thermocycle Conditions 5-21-13
2015-01-13
azim58 - Thermocycle Conditions 5-21-13 Thermocycle Conditions 5-21-13 has been associated with hs tdwn dyn (hot start touchdown dynazyme) protocol 95 C 30 s, 95 C hold, 95 C 2 min, 95 C hold, 95 C 2 min, 95 C 30 s, 65 C 30 s, 72 C 120 s, 95 C 30 s, 64 C 30 s, 72 C 120 s, 95 C 30 s, 63 C 30 s, 72 C 120 s, 95 C 30 s, 62 C 30 s, 72 C 120 s, 95 C 30 s, 61 C 30 s, 72 C 120 s, 95 C 30 s, 60 C 30 s, 72 C 120 s, 95 C 30 s, 59 C 30 s, 72 C 120 s, 95 C 30 s, 58 C 30 s, 72 C 120 s, 95 C 30 s, 57 C 30 s, 72 C 120 s, 95 C 30 s, 56 C 30 s, 72 C 120 s, (95 C 30 s, 55 C 30 s, 72 C 120 s)X40, 72 C 5 m, 4 C hold 1st part to warm up thermocycler 2nd part to denature 3rd part to add polymerase and denature further 10 decreasing annealing temperature cycles for touchdown amplification for 40 cycles
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