TentaGel

2015-01-13

azim58 - TentaGel

Tentagel S NH2 (90 micron mesh size beads)

density of peptide on bead is approximately 0.2-0.3 mmol/(g of Tentagel
resin)
90 micron mesh size of beads corresponds to 167 um (diameter?)
http://www.rapp-polymere.com/index.php?id=1220

number of beads per gram is 2.86*10^6 beads
http://www.rapp-polymere.com/index.php?id=1219

* before I found this information on the website I tried to determine
  this myself. I weighed out 0.912 mg into a 1.5 mL tube, diluted to 500
  uL, and then counted the beads in 10 uL fractions 3 times to get 31.3
  beads/10uL -> 31.3 beads/10uL*500uL = 1567 beads in total 500 uL volume
  -> 0.912mg/1567beads = 5.82*10^(-4) mg/bead or 582 ng/bead
* Number of beads in 1 g -> 1g*1*10^9ng/1g*1bead/582*ng = 1.72*10^6
  beads

swelling volume of Tentagel S beads in water is 3.6 mL/g
http://www.rapp-polymere.com/index.php?id=1218

Density of peptides bound to tentagel beads
0.25 mmol/1g*1g/3.6mL = 0.069 mmol/mL or 69 umol/mL
69 umol/dm^3*(10dm)^3/(1m)^3*1mol/1e6umol = 0.069 mol/m^3
One face of this cube would correspond to a certain density of peptide
per area
(x*mol/m)^3 =0.069*mol/m^3

* Actually this might be an improved calculation of the peptide
  density. I can determine the amount of peptide on one bead. I can then
  also determine the surface area of the outside of the bead as well as
  the surface area inside of the bead, and then calculate the amount of
  peptide per surface area.
  o How many peptides are there on one bead
    - 0.25mmol/1g*1mol/1000mmol*6.02e23/1mol*1g/2.86e6beads =
      5.26e13 peptides/bead

o The density of peptides will depend on the surface area.
    - For example
      5.26e13peptides/(0.10*4/3*Pi*(83.5)^3)*(1um)^2/(1000nm)^2 = 216
      peptides/nm^2
    - The larger the surface area is the lower the density of the
      peptides will be.
    - I need a good method for estimating the surface area of the
      Tentagel beads. I e-mailed the company Rapp Polymere to see if they
      have any information on this.
      @ https://mail.google.com/mail/u/0/?ui=2&amp;shva=1#sent/1384
        f3919fed65f1
      @ The company had no data available.
      @ Zhao says that when we synthesize the beads with two
        layers of lysine then the distance between the peptides will be 1
        nm, which is about the same as the distance between the peptides
        on the aminosilane array.

General Info about TentaGel resins
http://www.rapp-polymere.com/index.php?id=1215

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Orientation of Peptides Synthesized onto Tentagel Beads

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