TRF1 portal 09-25-2014d1413

2015-04-16

Issues to investigate
-TRF1 overexpression
-TRF1 deletion
-also look at TRF2

-[cni_TRF1 and TRF1 lox dna sequence details 04-12-2015d1006]

Mice with decreased TRF1 expression live longer, and they don't know why.  How much longer do they live?  No expression seems to be very bad.

Good paper to look at
-[TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells]
--recommended by Marinela

Some highlights
-A component of the shelterin complex
-Deficiency leads to embryonic lethality
-Deficiency leads to atrophy when deleted in adults
-Deficiency can impair tumor growth
-Highly expressed in stem cell compartments
-Highly expressed in iPSCs and also necessary for reprogramming
-TRF1 overexpression negatively regulates telomere length
--[cni_contradiction about TRF1 telomere length negative regulation and reprogramming 01-26-2015d1737]

TRF1 papers
-[Conditional TRF1 knockout in the hematopoietic compartment leads to bone marrow failure and recapitulates clinical features of Dyskeratosis congenita]
-[Increased telomere fragility and fusions resulting from TRF1 deficiency lead to degenerative pathologies and increased cancer in mice]
-[TRF1 controls telomere length and mitotic fidelity in epithelial homeostasis]

TRF1 review papers
{
TRF1, a mammalian telomeric protein
1997

Telomere structure and telomerase in health and disease (Review) 2012

Telomerase and telomere-associated proteins: structural insights into mechanism and evolution.
2012

Shelterin complex and associated factors at human telomeres.
2011

Inside the mammalian telomere interactome: regulation and regulatory activities of telomeres.
2006;16(2):103-18. Review

Shelterin: the protein complex that shapes and safeguards human telomeres. de Lange

Regulation of telomerase by telomeric proteins.

Telomeres in cancer and ageing. Blasco 2011 paper
}

RefSeq Mouse Terf1 TRF1 RNA
NM_001286628.1
http://www.ncbi.nlm.nih.gov/nuccore/NM_001286628

Primers for TRF1 RNA
RflExonControl_Fw       GTCTCTGTGCCGAGCCTTC     TRF1    Schneider, 2013 110bp   mouse (I don't understand where I got the name RflExonControl for these primers.  In Ralph's paper they have the name mTRF1.  I may have been confused.)
RflxExonControl_Rv      TCAATTGGTAAGCTGTAAGTCTGTG       TRF1                    mouse
TRF1BF  TCTAAGGATAGGCCAGATGCCA  TRF1    Muñoz,  185 bp  mouse
TRF1BR  CTGAAATCTGATGGAGCACGT   TRF1                    mouse
eGFP—TRF1—F
CCTGAGCAAAGACCCCAAC
eGFP—TRF1—R
TCCTCCTGCTCTGGAGAATC
-[cni_TRF1 primers of Agueda 03-29-2015d1129]

-[cni_Antibody for detecting TRF1-eGFP 03-12-2015d1032]

Some questions
What is the size of TRF1-eGFP protein?
-molecular weight is about 50 kD
--see Figure 7C of western blot in Ralph Schneider's Thesis

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