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T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12
2015-01-13
azim58 - T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12 T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12 (files stored at 1-5-12) Robosep Kit Used: Negative Selection Mouse T Cell Enrichment Kit 19751 Lot 11F39752 Antibodies used for Flow Cytometry: Product Information Sheets for Antibodies for Detecting T Cells 12-14-11 Protocols Followed: Splenocyte Preparation Protocol Purification of T cells with Robosep Kit Antibody Staining Protocol. The product sheets for the antibodies don't give very much information, but I would imagine the protocol would be very similar to the Annexin-V antibody Annexin V and PI or 7-AAD protocol Flow Cytometry * Some flow cytometry settings o FS, Volts 635, Gain 5.0 o SS, Volts 431, Gain 50.0
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