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Start process to construct transcript specific cDNA for blunt end cloning and sequencing

2015-01-13

azim58 - Start process to construct transcript specific cDNA for blunt end cloning and sequencing Start process to construct transcript specific cDNA for blunt end cloning and sequencing associated plan Plan for validating cDNA clones portal 1-7-13 Here are parts of the short names for the primers for the transcripts I would like to amplify RS61 RW17 RCX3 RRS8 REA RR130 RR130WT RH1 RM1 Rco1 refer to primers Tumor Validation primers 1-28-13 I will just use much less of the 5' oligo to ensure that I have enough to do the reaction (maybe I'll use 1/4). =========================================================================== The PCR products were very smeared on the gel (see 2-13-12 of notebook). I think I may need to use the RACE technique if I want to know the sequences of these transcripts.
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